SrasV1, Main, Exploration, bibRecord, 004689

Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Identifieur interne : 004689 ( Main/Exploration ); précédent : 004688; suivant : 004690

Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Auteurs : Bin Wang ; Simon J. Potter ; Yiguang Lin ; Anthony L. Cunningham ; Dominic E. Dwyer ; Yuelong Su ; Xuejun Ma ; Yunde Hou ; Nitin K. Saksena

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2005.

RBID : PMC:1153787

Descripteurs français

KwdFr :
- ADN viral (génétique), ARN viral (génétique), ARN viral (isolement et purification), Amorces ADN, Amplification de gène, Chine (épidémiologie), Données de séquences moléculaires, Humains, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (mortalité), Séquence nucléotidique, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : ADN viral, ARN viral, Virus du SRAS.
- isolement et purification : ARN viral, Virus du SRAS.
- mortalité : Syndrome respiratoire aigu sévère.
- épidémiologie : Chine.
- Amorces ADN, Amplification de gène, Données de séquences moléculaires, Humains, Sensibilité et spécificité, Séquence nucléotidique.
Wicri :
- geographic : République populaire de Chine.

English descriptors

KwdEn :
- Base Sequence, China (epidemiology), DNA Primers, DNA, Viral (genetics), Gene Amplification, Humans, Molecular Sequence Data, RNA, Viral (genetics), RNA, Viral (isolation & purification), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (mortality).
MESH :
- chemical , genetics : DNA, Viral, RNA, Viral.
- chemical , isolation & purification : RNA, Viral.
- chemical : DNA Primers.
- geographic , epidemiology : China.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- mortality : Severe Acute Respiratory Syndrome.
- Base Sequence, Gene Amplification, Humans, Molecular Sequence Data, Sensitivity and Specificity.

Abstract

The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (n = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153787

DOI: 10.1128/JCM.43.5.2339-2344.2005
PubMed: 15872263
PubMed Central: 1153787

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (<italic>n</italic>
 = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.</p>
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Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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